Wash your hands, put on your nightgown; look not so pale.—I tell you yet again, Banquo's buried; he cannot come out on's grave. Doctor: Even so?
This specific line refers back to the night of King Duncan's murder, specifically the moment when a knock at the door forced the couple to hurry to their chamber to avoid suspicion. Draft of the Full Speech Excerpt come_give_me_your_hand
This line highlights Lady Macbeth's transition from a cold, calculating figure to one "unhinged" by trauma and guilt. Wash your hands, put on your nightgown; look not so pale